Fluorescein isothiocyanates: Improved synthesis and purity

Synthesis and Applicationof New Fluorescein Analogues

In this project, new derivatives of fluorescein were synthesized using the reaction of maleic anhydride and saccharin with phenol derivatives in the presence of ZnCl2, and their structures were elucidated by UV, IR, and NMR spectroscopy. Fluorimetry studies showed that, the synthesized compounds can be utilized as fluorescent agents, and their efficiencies were dependent on pH of solution. Acco...

Isothiocyanates (XXIV)* Synthesis and Identification of 4-(/>-Terphenylyl)isothiocyanates and Their Intermediates

This paper deals with the synthesis of three up to date not described 4-(£>-terphenylyl)isothiocyanates and their six intermediates, two of which having not been described, too. A procedure was developed to identify £>-terphenyl derivatives by means of thin layer chromatography on alumina, as well as by gas chromatography. Infra-red and ultra-violet spectra were taken of the synthetized 4-(30-t...

synthesis and applicationof new fluorescein analogues

Fluorescein colchicine. Synthesis, purification, and biological activity

The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of ...

Synthesis and herbicidal activity of substituted pyrazole isothiocyanates.

Isothiocyanates and substituted pyrazoles were combined to form a series of novel isothiocyanates with highly effective herbicidal activity. The target compounds were analyzed by elemental analysis, 1H-NMR, EI-MS and IR spectroscopy. The synthesized compounds, particularly compounds 3-1 and 3-7, exhibited good herbicidal activities against four weeds. The EC(50) values of compound 3-1 against E...